我們的試劑品牌很多,都是高品質(zhì)的試劑
Recombinant human IL-6 protein, C-His產(chǎn)品編號bs-41177P英文名稱Recombinant human IL-6 protein, C-His中文名稱重組人白介素6蛋白別 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6. ;Recombinant human IL-6 protein, C-His Specific References (1) | bs-41177P has been referenced in 1 publications.[IF=4.966] 222Xiaolong Zhang. et al. Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. Cancer Sci. 2020 Oct; 111(10): 3802–3812 other ; PubMed:32691974理論分子量15kDa檢測分子量21 kDa性 狀Lyophilized or Liquid濃 度>0.5 mg/ml物 種Human序 列30-212/212純 度>95% as determined by SDS-PAGE純化方法AC內(nèi)毒素Not analyzed表達(dá)系統(tǒng)E.coli活性Not tested標(biāo)簽C-His緩 沖 液20 mM Tris-HCl (pH=8.0) with 150 mM NaCl保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項(xiàng)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae.SWISS:P05231Gene ID:3569產(chǎn)品圖片
Recombinant mouse IL-6 protein, C-His產(chǎn)品編號bs-10861P英文名稱Recombinant mouse IL-6 protein, C-His中文名稱重組小鼠白介素IL-6蛋白別 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6; Recombinant mouse IL-6 protein, C-His 理論分子量23kDa檢測分子量26 kDa性 狀Lyophilized or Liquid濃 度>0.5 mg/ml物 種Mouse序 列25-211/211純 度>95% as determined by SDS-PAGE純化方法AC內(nèi)毒素Not analyzed表達(dá)系統(tǒng)E.coli活性Not tested標(biāo)簽C-His緩 沖 液20 mM Tris-HCl with 20% Glycerol保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項(xiàng)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].SWISS:P08505Gene ID:16193產(chǎn)品圖片
Recombinant human IL-22 protein產(chǎn)品編號bs-43572P英文名稱Recombinant human IL-22 protein中文名稱重組人白介素22蛋白性 狀Lyophilized or Liquid濃 度1mg/ml保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項(xiàng)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹產(chǎn)品圖片
Recombinant human IL-10 protein (Active, HEK293) 產(chǎn)品編號ck0006英文名稱Recombinant human IL-10 protein (Active, HEK293)中文名稱重組人白介素10 (活性的, Tag free)別 名Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) 人白介素10 重組蛋白; 人白細(xì)胞介素10 ; 重組人白介素10; 重組人IL-10;理論分子量17kDa檢測分子量21 kDa性 狀Lyophilized powder濃 度>0.5 mg/ml物 種Human序 列19-178/178純 度>95% as determined by SDS-PAGE純化方法AC內(nèi)毒素<0.5 EU/μg as determined by LAL.表達(dá)系統(tǒng)HEK293 cell活性Yes標(biāo)簽Tag free緩 沖 液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.注意事項(xiàng)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹Interleukin 10(IL-10) is a member of the IL-10 family of α-helical cytokines. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of a number of proinflammatory cytokines such as IFNgamma, IL-2, IL-3, TNF and GM-CSF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.This recombinant human IL-10 is produced by human cells.(Tag free)Biological activity:The activity was measured by its ability to stimulate the proliferation of MC/9-2 cells (mouse master cell line).Reconstitution:Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.SWISS:P18893Gene ID:3586產(chǎn)品圖片
Recombinant human IL-15 protein (Active, HEK293) 產(chǎn)品編號ck0013產(chǎn)品分類重組蛋白英文名稱Recombinant human IL-15 protein (Active, HEK293)中文名稱重組人白介素15 (活性的, Tag free)英文別名rhIL-15; Interleukin-15; IL 15; Interleukin 15; Interleukin15; MGC9721; IL15; IL15_HUMAN. ;Recombinant human IL-15 protein (Active, HEK293)中文別名人白介素15重組蛋白; 重組人白介素15; 人重組白介素15;純化方法AC檢測分子量14-15 kDaGeneID3600SwissP40933濃度>0.5 mg/ml儲存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationIsoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.Tissue SpecificityMost abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.SimilarityBelongs to the IL-15/IL-21 family.Database linksEntrez Gene: 3600 HumanOmim: 600554 HumanSwissProt: P40933 HumanUnigene: 654378 Human多肽活性Yes蛋白質(zhì)與多肽-多肽序列49-162/162小分子偶聯(lián)蛋白-蛋白載體標(biāo)簽Tag free純度>95% as determined by SDS-PAGE內(nèi)毒素<0.5 EU/μg as determined by LAL.重組蛋白-表達(dá)系統(tǒng)HEK293 cell復(fù)溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
產(chǎn)品編號ck0017產(chǎn)品分類重組蛋白英文名稱Recombinant human IL-2 protein (Active, HEK293)中文名稱重組人白介素2 (活性的, Tag free)英文別名Interleukin-2; Interleukin 2; Interleukin2; rhIL-2; T-cell growth factor; T Cell Growth Factor; Aldesleukin; IL 2; IL2; Lymphokine; POIL2; T Cell Growth Factor; TCGF; Involved in regulation of T cell clonal expansion; IL2_HUMAN. ;Recombinant human IL-2 protein (Active, HEK293)中文別名人白介素2重組蛋白; 重組人白介素2; 人重組白介素2; 重組人白介素2(IL-2);蛋白細(xì)胞定位分泌型蛋白純化方法AC理論分子量15kDa檢測分子量17 kDaGeneID3558SwissP60568濃度>0.5 mg/ml儲存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationSecreted.DISEASENote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.SimilarityBelongs to the IL-2 family.Database linksEntrez Gene: 3558 HumanEntrez Gene: 16183 MouseEntrez Gene: 116562 RatOmim: 147680 HumanSwissProt: P60568 HumanSwissProt: P04351 MouseSwissProt: P17108 RatUnigene: 89679 HumanUnigene: 14190 MouseUnigene: 9871 Rat背景資料The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]多肽活性Yes蛋白質(zhì)與多肽-多肽序列21-153/153小分子偶聯(lián)蛋白-蛋白載體標(biāo)簽Tag free純度>95% as determined by SDS-PAGE內(nèi)毒素<0.5 EU/μg as determined by LAL.重組蛋白-表達(dá)系統(tǒng)HEK293 cell復(fù)溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Human IL-15(Interleukin 15) ELISA Kit文獻(xiàn)(2) 產(chǎn)品貨號:E-EL-H0222c 中文名:人白介素15(IL-15)酶聯(lián)免疫吸附測定試劑盒別稱:IL15用途 該試劑盒用于體外定量檢測Human 血清,血漿或其他生物體液中IL-15濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人IL-15抗體包被于酶標(biāo)板上,實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的人IL-15會與包被抗體結(jié)合,游離的成分被洗去。依次加入生物素化的抗人IL-15抗體和辣根過氧化物酶標(biāo)記的親和素。抗人IL-15抗體與結(jié)合在包被抗體上的人IL-15結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物,游離的成分被洗去。加入顯色底物(TMB),TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色,加終止液后變成黃色。用酶標(biāo)儀在450nm波長處測OD值,IL-15濃度與OD450值之間呈正比,通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中IL-15的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時(shí)間 3.5h反應(yīng)性 Human檢測方法 Colormetric檢測范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清,血漿或其他生物體液特異性 可檢測樣本中的人IL-15,且與其它相關(guān)蛋白無明顯交叉反應(yīng)。重復(fù)性 板內(nèi),板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實(shí)驗(yàn)操作條件的不同(如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等),標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考,實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 10002.4852.5412.5132.4365001.6481.6681.6581.5812500.9160.8840.90.8231250.480.50.490.41362.50.2910.2710.2810.20431.250.1790.1770.1780.10115.630.1240.1340.1290.05200.0700.0840.077--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 47.35146.58348.0446.47145.32338.28Standarddeviation 3.028.2715.043.166.4718.37C V (%) 6.385.644.326.804.455.43回收率 分別往5個(gè)不同樣本中添加已知濃度的目標(biāo)蛋白,做回收實(shí)驗(yàn),得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 92-10698EDTA plasma (n=8) 92-10397Cell culture media (n=8) 88-10394線性 分別往5個(gè)樣本中添加已知濃度的目標(biāo)蛋白,做回收實(shí)驗(yàn),得出回收率范圍及平均回收率。將5個(gè)樣本分別稀釋2倍,4倍,8倍,16倍做回收實(shí)驗(yàn),得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)96-11096-10984-97Average (%)101102901:4Range (%)88-10183-9584-96Average (%)9588911:8Range (%)91-10581-9289-99Average (%)9987941:16Range (%)93-10488-10088-100Average (%)999394試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周;如果一周以后才使用試劑盒,請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃,可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光),可存放6個(gè)月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個(gè)月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報(bào)告Certificate of Analysis1 份說明:所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實(shí)際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時(shí)會比標(biāo)簽上標(biāo)明的體積稍多一些,請?jiān)谑褂脮r(shí)量取而非直接倒出。試驗(yàn)所需自備物品 1. 酶標(biāo)儀(450nm波長濾光片)2. 高精度移液器,EP管及一次性吸頭:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱,雙蒸水或去離子水4. 吸水紙操作步驟1. 對應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本,37℃孵育90分鐘2. 棄掉板內(nèi)液體后,立即加入100μL生物素化抗體工作液,37℃孵育60分鐘3. 棄掉板內(nèi)液體,洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液,37℃孵育30分鐘,棄掉板內(nèi)液體,洗板5次5. 每孔加入90μL底物溶液,37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數(shù),處理數(shù)據(jù)
Human IL-23(Interleukin 23) ELISA Kit文獻(xiàn)(10) 產(chǎn)品貨號:E-EL-H0107c 中文名:人白介素23(IL-23)酶聯(lián)免疫吸附測定試劑盒別稱:IL-23A, IL23P19, P19, SGRF用途 該試劑盒用于體外定量檢測Human 血清,血漿或其他生物體液中IL-23濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人IL-23抗體包被于酶標(biāo)板上,實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的人IL-23會與包被抗體結(jié)合,游離的成分被洗去。依次加入生物素化的抗人IL-23抗體和辣根過氧化物酶標(biāo)記的親和素。抗人IL-23抗體與結(jié)合在包被抗體上的人IL-23結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物,游離的成分被洗去。加入顯色底物(TMB),TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色,加終止液后變成黃色。用酶標(biāo)儀在450nm波長處測OD值,IL-23濃度與OD450值之間呈正比,通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中IL-23的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時(shí)間 3.5h反應(yīng)性 Human檢測方法 Colormetric檢測范圍 39.06—2500 pg/mL靈敏度 23.44 pg/mL樣本體積 100μL樣本類型 血清,血漿或其他生物體液特異性 可檢測樣本中的人IL-23,且與其它相關(guān)蛋白無明顯交叉反應(yīng)。重復(fù)性 板內(nèi),板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實(shí)驗(yàn)操作條件的不同(如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等),標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考,實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 25002.1472.1892.1682.11512501.4851.5191.5021.4496250.830.7920.8110.758312.50.3640.3740.3690.316156.250.230.2020.2160.16378.130.1480.1320.140.08539.060.0930.1050.0990.04600.0450.0610.053--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 136.57343.261072.26129.52356.171144.30Standarddeviation 7.2015.5535.498.4820.0254.47C V (%) 5.274.533.316.555.624.76回收率 分別往5個(gè)不同樣本中添加已知濃度的目標(biāo)蛋白,做回收實(shí)驗(yàn),得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 88-10094EDTA plasma (n=8) 94-10599Cell culture media (n=8) 87-10093線性 分別往5個(gè)樣本中添加已知濃度的目標(biāo)蛋白,做回收實(shí)驗(yàn),得出回收率范圍及平均回收率。將5個(gè)樣本分別稀釋2倍,4倍,8倍,16倍做回收實(shí)驗(yàn),得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)92-10795-10985-99Average (%)99102911:4Range (%)93-10484-9686-102Average (%)9890931:8Range (%)91-10683-9487-99Average (%)9989941:16Range (%)88-10384-9480-92Average (%)958987試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周;如果一周以后才使用試劑盒,請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃,可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光),可存放6個(gè)月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個(gè)月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報(bào)告Certificate of Analysis1 份說明:所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實(shí)際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時(shí)會比標(biāo)簽上標(biāo)明的體積稍多一些,請?jiān)谑褂脮r(shí)量取而非直接倒出。試驗(yàn)所需自備物品 1. 酶標(biāo)儀(450nm波長濾光片)2. 高精度移液器,EP管及一次性吸頭:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱,雙蒸水或去離子水4. 吸水紙操作步驟1. 對應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本,37℃孵育90分鐘2. 棄掉板內(nèi)液體后,立即加入100μL生物素化抗體工作液,37℃孵育60分鐘3. 棄掉板內(nèi)液體,洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液,37℃孵育30分鐘,棄掉板內(nèi)液體,洗板5次5. 每孔加入90μL底物溶液,37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數(shù),處理數(shù)據(jù)
Human sIL-6R(Soluble Interleukin 6 Receptor) ELISA Kit產(chǎn)品貨號:E-EL-H2518c 中文名:人可溶性白介素6受體(sIL-6R)酶聯(lián)免疫吸附測定試劑盒別稱:IL6R, CD126, IL-6R-1, IL-6RA, IL6Q, IL6RA, IL6RQ, gp80, Interleukin-6 receptor, interleukin 6 receptor用途 該試劑盒用于體外定量檢測Human 血清,血漿或其他生物體液中sIL-6R濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人sIL-6R抗體包被于酶標(biāo)板上,實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的人sIL-6R會與包被抗體結(jié)合,游離的成分被洗去。依次加入生物素化的抗人sIL-6R抗體和辣根過氧化物酶標(biāo)記的親和素。抗人sIL-6R抗體與結(jié)合在包被抗體上的人sIL-6R結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物,游離的成分被洗去。加入顯色底物(TMB),TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色,加終止液后變成黃色。用酶標(biāo)儀在450nm波長處測OD值,sIL-6R濃度與OD450值之間呈正比,通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中sIL-6R的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時(shí)間 4.5h反應(yīng)性 Human檢測方法 Colormetric檢測范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清,血漿或其他生物體液特異性 可檢測樣本中的人sIL-6R,且與其它相關(guān)蛋白無明顯交叉反應(yīng)。重復(fù)性 板內(nèi),板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實(shí)驗(yàn)操作條件的不同(如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等),標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考,實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 10002.4172.4692.4432.3845001.6951.7411.7181.6592500.9740.9390.9570.8981250.4350.4550.4450.38662.50.2680.2460.2570.19831.250.1750.1490.1620.10315.630.1060.1180.1120.05300.0490.0690.059--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 54.13124.28477.0256.05115.03491.14Standarddeviation 3.265.4422.943.755.2221.71C V (%) 6.024.384.816.694.544.42回收率 分別往5個(gè)不同樣本中添加已知濃度的目標(biāo)蛋白,做回收實(shí)驗(yàn),得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 83-9590EDTA plasma (n=8) 87-9792Cell culture media (n=8) 83-9590線性 分別往5個(gè)樣本中添加已知濃度的目標(biāo)蛋白,做回收實(shí)驗(yàn),得出回收率范圍及平均回收率。將5個(gè)樣本分別稀釋2倍,4倍,8倍,16倍做回收實(shí)驗(yàn),得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)87-9998-11196-110Average (%)931051031:4Range (%)86-100101-11698-111Average (%)921071041:8Range (%)84-9797-10894-111Average (%)891021021:16Range (%)83-92100-11792-107Average (%)88108100試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周;如果一周以后才使用試劑盒,請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃,可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光),可存放6個(gè)月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個(gè)月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報(bào)告Certificate of Analysis1 份說明:所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實(shí)際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時(shí)會比標(biāo)簽上標(biāo)明的體積稍多一些,請?jiān)谑褂脮r(shí)量取而非直接倒出。試驗(yàn)所需自備物品 1. 酶標(biāo)儀(450nm波長濾光片)2. 高精度移液器,EP管及一次性吸頭:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱,雙蒸水或去離子水4. 吸水紙操作步驟1. 對應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本,37℃孵育90分鐘2. 棄掉板內(nèi)液體后,立即加入100μL生物素化抗體工作液,37℃孵育60分鐘3. 棄掉板內(nèi)液體,洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液,37℃孵育30分鐘,棄掉板內(nèi)液體,洗板5次5. 每孔加入90μL底物溶液,37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數(shù),處理數(shù)據(jù)
Uncoated Human IL-6(Interleukin 6) ELISA KitUniprot : P05231 中文名稱:未包被人白介素6(IL-6)酶聯(lián)免疫吸附測定試劑盒產(chǎn)品貨號: E-UNEL-H0097 實(shí)驗(yàn)類型: Sandwich-ELISA Applications:ELISA 產(chǎn)品用途 Elabscience?新系列未包被(Uncoated)ELISA試劑盒,包含用于ELISA實(shí)驗(yàn)所需的基本組分。對于具有豐富經(jīng)驗(yàn),可自行建立ELISA實(shí)驗(yàn)體系的客戶,未包被試劑盒是其節(jié)省成本的理想選擇。可用于體外定量檢測人血清,血漿中IL-6濃度。產(chǎn)品屬性 反應(yīng)類型夾心法規(guī)格96T*5 96T*15反應(yīng)時(shí)間24h反應(yīng)性人保存條件-20℃。請按照說明書要求儲存試劑盒。樣本類型血清,血漿樣本體積100μL產(chǎn)品組成 中文名稱 英文名稱 規(guī)格 保存條件 ELISA預(yù)制酶標(biāo)板(可拆卸)Micro ELISA pre-Plate(Dismountable)96T*5:5 塊 96T酶標(biāo)板-20℃,可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard96T*5:1支濃縮包被抗體Concentrated Capture Ab96T*5:1 支 120μL濃縮生物素化抗體Concentrated Biotinylated Detection Ab96T*5:1 支 120μL濃縮HRP酶結(jié)合物Concentrated HRP Conjugate96T*5:1 支 120μL-20℃(避光),可存放6個(gè)月產(chǎn)品說明書Product Description1 份/質(zhì)檢報(bào)告Certificate of Analysis1 份實(shí)驗(yàn)原理 1. 預(yù)制酶標(biāo)板包被上分析物特異性抗體(包被抗體),未結(jié)合的包被抗體被洗脫。后完成封閉操作,得到預(yù)包被酶標(biāo)板。2. 加入的樣本或標(biāo)準(zhǔn)品將被固定的包被抗體所捕獲,未被捕獲的成分將被移除。3. 加入的生物素化檢測抗體將結(jié)合已被包被抗體捕獲的分析物,未結(jié)合的成分將被洗脫。4. 加入HRP酶結(jié)合物結(jié)合生物素化檢測抗體,未結(jié)合的成分將被洗脫。5. 加入TMB底物溶液,藍(lán)色與樣品中存在的分析物的量成比例。加入終止液后,底物顯色變化終止,孔內(nèi)顏色由藍(lán)色變成黃色。測量450nm處各孔的吸光度值。
Human CGRP1(Calcitonin Gene Related Peptide 1) ELISA Kit文獻(xiàn)(3) 產(chǎn)品貨號:E-EL-H0619c 中文名:人降鈣素基因相關(guān)肽1(CGRP1)酶聯(lián)免疫吸附測定試劑盒別稱:CGRP1用途 該試劑盒用于體外定量檢測Human 血清,血漿或其他生物體液中CGRP1濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人CGRP1抗體包被于酶標(biāo)板上,實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的人CGRP1會與包被抗體結(jié)合,游離的成分被洗去。依次加入生物素化的抗人CGRP1抗體和辣根過氧化物酶標(biāo)記的親和素。抗人CGRP1抗體與結(jié)合在包被抗體上的人CGRP1結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物,游離的成分被洗去。加入顯色底物(TMB),TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色,加終止液后變成黃色。用酶標(biāo)儀在450nm波長處測OD值,CGRP1濃度與OD450值之間呈正比,通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中CGRP1的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時(shí)間 3.5h反應(yīng)性 Human檢測方法 Colormetric檢測范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清,血漿或其他生物體液特異性 可檢測樣本中的人CGRP1,且與其它相關(guān)蛋白無明顯交叉反應(yīng)。重復(fù)性 板內(nèi),板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實(shí)驗(yàn)操作條件的不同(如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等),標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考,實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 10002.5522.5822.5672.4835001.6631.7131.6881.6042500.9590.9380.9490.8651250.5080.5240.5160.43262.50.30.2740.2870.20331.250.1910.1830.1870.10315.630.130.1440.1370.05300.0810.0860.084--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 48.15145.75349.4351.54133.87361.48Standarddeviation 3.108.3113.663.406.8413.59C V (%) 6.445.703.916.605.113.76回收率 分別往5個(gè)不同樣本中添加已知濃度的目標(biāo)蛋白,做回收實(shí)驗(yàn),得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 89-10396EDTA plasma (n=8) 87-9892Cell culture media (n=8) 91-10596線性 分別往5個(gè)樣本中添加已知濃度的目標(biāo)蛋白,做回收實(shí)驗(yàn),得出回收率范圍及平均回收率。將5個(gè)樣本分別稀釋2倍,4倍,8倍,16倍做回收實(shí)驗(yàn),得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)83-9889-10294-107Average (%)90941011:4Range (%)86-10084-9589-102Average (%)9289941:8Range (%)90-10384-9989-102Average (%)9691951:16Range (%)92-10686-9787-98Average (%)989292試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周;如果一周以后才使用試劑盒,請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃,可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光),可存放6個(gè)月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個(gè)月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報(bào)告Certificate of Analysis1 份說明:所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實(shí)際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時(shí)會比標(biāo)簽上標(biāo)明的體積稍多一些,請?jiān)谑褂脮r(shí)量取而非直接倒出。試驗(yàn)所需自備物品 1. 酶標(biāo)儀(450nm波長濾光片)2. 高精度移液器,EP管及一次性吸頭:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱,雙蒸水或去離子水4. 吸水紙操作步驟1. 對應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本,37℃孵育90分鐘2. 棄掉板內(nèi)液體后,立即加入100μL生物素化抗體工作液,37℃孵育60分鐘3. 棄掉板內(nèi)液體,洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液,37℃孵育30分鐘,棄掉板內(nèi)液體,洗板5次5. 每孔加入90μL底物溶液,37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數(shù),處理數(shù)據(jù)
產(chǎn)品簡介儲存條件2-25℃避光規(guī)格10*50ml 10*90ml 10*225ml單位盒產(chǎn)品用途 用于沙門氏菌的前增菌培養(yǎng)。檢驗(yàn)原理 蛋白胨提供碳源和氮源滿足細(xì)菌生長的需求;氯化鈉可維持均衡的滲透壓;磷酸二氫鉀和磷酸氫二鈉是緩沖劑。配方成分 配方(每升) 含量蛋白胨 10.0g氯化鈉 5.0g無水磷酸氫二鈉 3.57g磷酸二氫鉀 1.5g蒸餾水 1000mL最終 pH 7.2±0.2使用方法 拆包即可使用。
產(chǎn)品簡介儲存條件2~8℃,避光規(guī)格10*225ml單位盒產(chǎn)品用途 可廣泛應(yīng)用于細(xì)菌的培養(yǎng),特別用于消毒劑消毒效果的測試、金黃色葡萄球菌的非選擇性增菌培養(yǎng)及蠟樣芽孢桿菌的多管發(fā)酵法測定。檢驗(yàn)原理 胰蛋白胨、植物蛋白胨提供氮源、維生素和生長因子;葡萄糖提供碳源;磷酸氫二鉀為緩沖劑;氯化鈉維持均衡的滲透壓;多粘菌素 B 可抑制雜菌的生長。配方成分 配方(每升) 含量胰蛋白胨 17.0g植物蛋白胨 3.0g磷酸氫二鉀 2.5g氯化鈉 5.0g葡萄糖 2.5g多黏菌素 B 100000UI蒸餾水 1000mL最終 pH 7.3±0.2使用方法 拆開包裝即可使用。