中文 
Novoprotein/近岸蛋白
Novoprotein/近岸蛋白
NovoRec? plus One step PCR Cloning Kit-100次
基本售價：2000.00元
產品描述一步定向克隆試劑盒是一款簡單、快速、高效的多片段DNA定向克隆產品，可以不依賴于連接酶，不受載體和目的片段的酶切位點限制，直接用同源重組的方法將PCR產物定向克隆至任意載體的任意位點，完成目的片段與載體相連接的基因克隆技術。該技術操作簡單，PCR產物一步定向克隆，目的片段與載體無縫連接，反應時間短至10分鐘，陽性率達到95%以上，讓您輕松完成實驗。保存條件收到后請立即低速離心保存在-20℃；此Kit可在-20℃長期保存，避免反復凍融。
NovoRec? plus One step PCR Cloning Kit-20次
基本售價：600.00元
產品描述一步定向克隆試劑盒是一款簡單、快速、高效的多片段DNA定向克隆產品，可以不依賴于連接酶，不受載體和目的片段的酶切位點限制，直接用同源重組的方法將PCR產物定向克隆至任意載體的任意位點，完成目的片段與載體相連接的基因克隆技術。該技術操作簡單，PCR產物一步定向克隆，目的片段與載體無縫連接，反應時間短至10分鐘，陽性率達到95%以上，讓您輕松完成實驗。保存條件收到后請立即低速離心保存在-20℃；此Kit可在-20℃長期保存，避免反復凍融。
NovoScript?Plus All-in-one 1st Strand cDNA Synthesis SuperMix-100次（20μl體系）
基本售價：1080.00元
產品描述第一鏈cDNA合成預混液是以mRNA或者總RNA為模板，高效合成第一鏈cDNA。本產品含有反轉錄反應所需的全部試劑（NovoScript? II Reverse Transcriptase，RNase Inhibitor，Oligo(dT)18，Random N6，dNTPs）。反應時只需要加入RNA模板和水即可，操作簡單，降低操作過程中的污染。制品中含有去基因組成分gDNA Purge，以RNA為模板進行cDNA第一鏈合成時，可以同步去除基因組DNA污染，反應結束后，75℃加熱5分鐘，就可以失活反轉錄酶，RNA酶抑制劑。合成的第一鏈cDNA能直接用于PCR或熒光定量PCR的模板，第二鏈cDNA的合成或線性RNA擴增，也可用于需要用帶有放射性或非放射性核苷酸標記第一鏈cDNA的實驗。產品特點1)本制品采用耐高溫逆轉錄酶，大幅提高熱穩定性和cDNA合成效率；2)本制品中添加的NovoScript? II Reverse Transcriptase無RNase H活性，可避免第一鏈cDNA合成過程中，RNA/DNA雜交模板鏈中RNA降解，保證cDNA合成的質量；3)本制品中Random N6和Oligo(dT)18引物比例經過優化，可均勻合成樣品中的cDNA；4)本制品中含有去基因組成分，逆轉錄時可同步去除基因組DNA污染。保存條件-20℃。注意事項1)用DEPC處理實驗用到的所有實驗器材，或者購買已經證明無核酸酶的實驗用具，實驗過程戴手套并經常更換手套，避免RNA酶的污染。2)確保所用試劑中無RNA酶污染。3)試劑盒要嚴格密封保存。在逆轉錄過程中，所有管子要確?？蹏馈?)純化過的RNA必須保證不含有鹽，金屬離子，乙醇和苯酚，以上成分會干擾第一鏈cDNA的合成反應。可采用乙醇沉淀RNA的方法去除掉痕量污染物。5)為保證逆轉錄反應的有效進行，需使用高質量的RNA模板。6)當模板含量較低或后續PCR擴增片段過長時可以適當延長逆轉錄時間。7)2×NovoScript? Plus 1st Strand cDNA Synthesis SuperMix、gDNA Purge及2×NovoScript? Plus No RT Control含有甘油，使用前請充分混勻后并短暫離心收集至管底再進行使用。僅供科研或生產使用，不可直接應用于人體。
NovoScript?Plus All-in-one 1st Strand cDNA Synthesis SuperMix-50次（20μl體系）
基本售價：600.00元
產品描述第一鏈cDNA合成預混液是以mRNA或者總RNA為模板，高效合成第一鏈cDNA。本產品含有反轉錄反應所需的全部試劑（NovoScript? II Reverse Transcriptase，RNase Inhibitor，Oligo(dT)18，Random N6，dNTPs）。反應時只需要加入RNA模板和水即可，操作簡單，降低操作過程中的污染。制品中含有去基因組成分gDNA Purge，以RNA為模板進行cDNA第一鏈合成時，可以同步去除基因組DNA污染，反應結束后，75℃加熱5分鐘，就可以失活反轉錄酶，RNA酶抑制劑。合成的第一鏈cDNA能直接用于PCR或熒光定量PCR的模板，第二鏈cDNA的合成或線性RNA擴增，也可用于需要用帶有放射性或非放射性核苷酸標記第一鏈cDNA的實驗。產品特點1)本制品采用耐高溫逆轉錄酶，大幅提高熱穩定性和cDNA合成效率；2)本制品中添加的NovoScript? II Reverse Transcriptase無RNase H活性，可避免第一鏈cDNA合成過程中，RNA/DNA雜交模板鏈中RNA降解，保證cDNA合成的質量；3)本制品中Random N6和Oligo(dT)18引物比例經過優化，可均勻合成樣品中的cDNA；4)本制品中含有去基因組成分，逆轉錄時可同步去除基因組DNA污染。保存條件-20℃。注意事項1)用DEPC處理實驗用到的所有實驗器材，或者購買已經證明無核酸酶的實驗用具，實驗過程戴手套并經常更換手套，避免RNA酶的污染。2)確保所用試劑中無RNA酶污染。3)試劑盒要嚴格密封保存。在逆轉錄過程中，所有管子要確?？蹏?。4)純化過的RNA必須保證不含有鹽，金屬離子，乙醇和苯酚，以上成分會干擾第一鏈cDNA的合成反應?？刹捎靡掖汲恋鞷NA的方法去除掉痕量污染物。5)為保證逆轉錄反應的有效進行，需使用高質量的RNA模板。6)當模板含量較低或后續PCR擴增片段過長時可以適當延長逆轉錄時間。7)2×NovoScript? Plus 1st Strand cDNA Synthesis SuperMix、gDNA Purge及2×NovoScript? Plus No RT Control含有甘油，使用前請充分混勻后并短暫離心收集至管底再進行使用。僅供科研或生產使用，不可直接應用于人體。
NovoStart? SYBR High-Sensitivity qPCR SuperMix-1ml×5
基本售價：1280.00元
產品描述本制品是采用SYBR Green I嵌合熒光法進行Real Time PCR的專用試劑。預混液中已經將DNA聚合酶、精心優化的反應Buffer、dNTPs、SYBR Green I等試劑預混在一起，是一種2×濃度的預混型試劑，進行實驗時，PCR反應液的配制十分簡單方便。本制品使用新型抗體修飾的HotStart Taq DNA聚合酶，并結合精心優化的反應Buffer，具有更高的擴增靈敏度，反應特異性和擴增效率，能夠在更寬廣的范圍內進行準確定量。產品特點高靈敏度：Novoprotein精心配制的RealTime PCR專用2×SuperMix，具有更高的擴增靈敏度和擴增效率。高特異性：采用新型工藝制備抗體修飾的HotStart Taq DNA聚合酶，無需熱啟動即可進行PCR反應，大大提高PCR擴增的特異性?？旖荩篜CR反應所必需試劑集于一管之中，數分鐘即可完成反應體系配制。保存條件-20℃避光儲存。如需在一段時間內經常取用，可在2~8℃條件下儲存3個月。避免反復多次凍融。質量控制純度檢測：所有組分經檢測均無核酸內切酶殘留、核酸外切酶殘留、核酸殘留。使用建議使用：請上下顛倒輕輕混合均勻，避免起泡，并經輕微離心后使用。反應液的配制、分裝請使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應體積為20～50μl。引物設計：一般為了增加靈敏度及擴增效率且抑制非特異性反應，擴增目標序列越短越好。建議設計成200bp以下。僅供科研或生產使用，不可直接應用于人體。
NovoStart? SYBR High-Sensitivity qPCR SuperMix-1ml×25
基本售價：5670.00元
產品描述本制品是采用SYBR Green I嵌合熒光法進行Real Time PCR的專用試劑。預混液中已經將DNA聚合酶、精心優化的反應Buffer、dNTPs、SYBR Green I等試劑預混在一起，是一種2×濃度的預混型試劑，進行實驗時，PCR反應液的配制十分簡單方便。本制品使用新型抗體修飾的HotStart Taq DNA聚合酶，并結合精心優化的反應Buffer，具有更高的擴增靈敏度，反應特異性和擴增效率，能夠在更寬廣的范圍內進行準確定量。產品特點高靈敏度：Novoprotein精心配制的RealTime PCR專用2×SuperMix，具有更高的擴增靈敏度和擴增效率。高特異性：采用新型工藝制備抗體修飾的HotStart Taq DNA聚合酶，無需熱啟動即可進行PCR反應，大大提高PCR擴增的特異性。快捷：PCR反應所必需試劑集于一管之中，數分鐘即可完成反應體系配制。保存條件-20℃避光儲存。如需在一段時間內經常取用，可在2~8℃條件下儲存3個月。避免反復多次凍融。質量控制純度檢測：所有組分經檢測均無核酸內切酶殘留、核酸外切酶殘留、核酸殘留。使用建議使用：請上下顛倒輕輕混合均勻，避免起泡，并經輕微離心后使用。反應液的配制、分裝請使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應體積為20～50μl。引物設計：一般為了增加靈敏度及擴增效率且抑制非特異性反應，擴增目標序列越短越好。建議設計成200bp以下。僅供科研或生產使用，不可直接應用于人體。
NovoStart?SYBR qPCR SuperMix Plus-1ml×25
基本售價：5670.00元
產品描述本制品是采用SYBR Green I嵌合熒光法進行Real Time PCR的專用試劑。預混液中已經將DNA聚合酶、精心優化的反應Buffer、dNTPs、SYBR Green I等試劑預混在一起，是一種2×濃度的預混型試劑，進行實驗時，PCR反應液的配制十分簡單方便。本制品使用新型抗體修飾的HotStart Taq DNA聚合酶，并結合精心優化的反應Buffer，從而有效抑制低溫下的非特異性擴增，提高反應特異性和擴增效率，能夠在更寬廣的范圍內進行準確定量。產品特點高特異性：采用新型工藝制備抗體修飾的HotStart Taq DNA聚合酶，無需熱啟動即可進行PCR反應，大大提高PCR擴增的特異性。高效：Novoprotein精心配制的RealTime PCR專用2×SuperMix，具有更高的擴增效率和擴增靈敏度。快捷：PCR反應所必需試劑集于一管之中，數分鐘即可完成反應體系配制。保存條件-20℃避光儲存。如需在一段時間內經常取用，可在2~8℃條件下儲存3個月。避免反復多次凍融。質量控制純度檢測：所有組分經檢測均無核酸內切酶殘留、核酸外切酶殘留、核酸殘留。使用建議使用：請上下顛倒輕輕混合均勻，避免起泡，并經輕微離心后使用。反應液的配制、分裝請使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應體積為20～50μl。引物設計：一般為了增加靈敏度及擴增效率且抑制非特異性反應，擴增目標序列越短越好。建議設計成200bp以下。僅供科研或生產使用，不可直接應用于人體。
NovoStart? Fast SYBR qPCR SuperMix-1ml×25
基本售價：6336.00元
產品描述本制品是采用 SYBR Green I 嵌合熒光法進行快速 Real Time PCR 的 2×濃度預混型試劑，PCR 反應液的配制十分簡單方便。本制品使用突變型 Taq DNA 聚合酶，結合精心優化的反應 Buffer，可有效抑制低溫下的非特異性擴增，實現快速 PCR 反應，并且能夠在寬廣的范圍內對靶基因進行準確、快速、可重復的定量檢測。產品特點快速：實現快速熒光定量，相較于傳統 qPCR，反應時間減少 50%左右；保存條件-20℃避光儲存，避免反復凍融。使用建議使用：請上下顛倒輕輕混合均勻，避免起泡，并經輕微離心后使用。反應液的配制、分裝請使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應體積為20～50μl。僅供科研或生產使用，不可直接應用于人體。Novoprotein是蘇州近岸蛋白質科技股份有限公司的注冊商標。請訪問我們的注冊商標頁面。其他商標是其他所有者的財產。
NovoStart? Fast SYBR qPCR SuperMix-Imlx5
基本售價：1408.00元
產品描述本制品是采用 SYBR Green I 嵌合熒光法進行快速 Real Time PCR 的 2×濃度預混型試劑，PCR 反應液的配制十分簡單方便。本制品使用突變型 Taq DNA 聚合酶，結合精心優化的反應 Buffer，可有效抑制低溫下的非特異性擴增，實現快速 PCR 反應，并且能夠在寬廣的范圍內對靶基因進行準確、快速、可重復的定量檢測。產品特點快速：實現快速熒光定量，相較于傳統 qPCR，反應時間減少 50%左右；保存條件-20℃避光儲存，避免反復凍融。使用建議使用：請上下顛倒輕輕混合均勻，避免起泡，并經輕微離心后使用。反應液的配制、分裝請使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應體積為20～50μl。僅供科研或生產使用，不可直接應用于人體。Novoprotein是蘇州近岸蛋白質科技股份有限公司的注冊商標。請訪問我們的注冊商標頁面。其他商標是其他所有者的財產。
NovoNGS? CUT&Tag? 4.0 High-Sensitivity Kit (for Illumina?)-24次
基本售價：17400.00元
產品描述Cleavage Under Target & Tagmentation (CUT&Tag?)是一種新興的DNA-蛋白質互作研究方法。CUT&Tag? 以融合了protein A/G 的Tn5 轉座酶為核心，融合蛋白通過protein A/G 與抗體結合，使得Tn5 被栓系在靶位點周圍，從而在目的位點附近進行酶切反應，并同時引入二代測序所必需的接頭序列，產物DNA 經過后續提取與PCR 擴增后，得到直接用于測序的文庫。CUT&Tag?與傳統的ChIP-Seq 技術相比較，實驗不需要超聲打斷，也不需要傳統的連接法添加測序接頭，操作簡單、周期短、信噪比高、重復性好、細胞用量少，為極低起始細胞量和單細胞測序研究提供了可能性。自 2019 年 CUT&Tag 技術誕生以來，經過大量的實踐，發現該技術在組蛋白修飾及部分轉錄因子與 DNA 互作研究中可以獲得高信噪比的結果，但是針對一些與 DNA 結合力較弱或者結合具有高度動態性的 DNA 結合蛋白，較難得到理想的實驗結果。針對此問題，近岸蛋白開發了可對細胞進行高濃度甲醛交聯的CUT&Tag實驗流程。為轉錄因子 -DNA 互作研究提供一種新的思路，這種需要甲醛交聯的CUT&Tag實驗被稱為fcCUT&Tag（formaldehyde crosslinking CUT&Tag）。NovoNGS? CUT&Tag? 4.0 High-Sensitivity Kit (for Illumina）試劑盒包含常規非交聯細胞CUT&Tag及甲醛交聯細胞fcCUT&Tag實驗中所需的各種緩沖液、ConA磁珠、DNA純化磁珠、引物等全套建庫試劑，可以用于組蛋白修飾和轉錄因子的染色質結合狀態研究。與傳統的CUT&Tag試劑盒相比，該試劑盒既可以對自然狀態的細胞進行蛋白質-DNA互作研究，也可以對高濃度甲醛交聯的細胞進行蛋白質-DNA互作研究，一盒兩用。試劑盒包含CUT&Tag? 實驗中孵育抗體、轉座體、構建文庫的相關試劑，可以用于組蛋白修飾和轉錄因子的染色質結合狀態研究。本試劑盒包含高活性的ChiTag? pAG-Tn5, 相較于最初版本的pA-Tn5 轉座酶，pAG結構域對兔、鼠等多種來源抗體具有廣譜的強親和性，在實驗抗體的選擇上具有更大的空間。針對CUT&Tag?打斷產物DNA 提取過程中小片段丟失的問題，本試劑盒提供優化的Tagment DNA Extract Beads, 相較于常規的片段分選磁珠可以完整的回收CUT&Tag? 產物中的小片段DNA, 大大簡化了酚氯仿-乙醇沉淀法的繁瑣操作。此外，本試劑盒還提供了陽性抗體H3K4me3（兔抗）以及相應的二抗（羊抗兔IgG) ，用于陽性對照實驗（僅B 包裝提供陽性抗體）。圖1： DNA-Target Protein-Antibody-ChiTag?結合示意圖 圖2： CUT&Tag 實驗流程簡圖 產品用途DNA－蛋白質互作研究。在常見的哺乳動物細胞上用于替代傳統的ChIP-Seq 技術，特別適用于細胞數量較少的樣本，也可應用于經過處理的植物和酵母樣本上。保存條件詳見說明書僅供科研或生產使用，不可直接應用于人體。
NovoNGS? CUT&Tag? 4.0 High-Sensitivity Kit (for Illumina?)-12次
基本售價：9000.00元
產品描述Cleavage Under Target & Tagmentation (CUT&Tag?)是一種新興的DNA-蛋白質互作研究方法。CUT&Tag? 以融合了protein A/G 的Tn5 轉座酶為核心，融合蛋白通過protein A/G 與抗體結合，使得Tn5 被栓系在靶位點周圍，從而在目的位點附近進行酶切反應，并同時引入二代測序所必需的接頭序列，產物DNA 經過后續提取與PCR 擴增后，得到直接用于測序的文庫。CUT&Tag?與傳統的ChIP-Seq 技術相比較，實驗不需要超聲打斷，也不需要傳統的連接法添加測序接頭，操作簡單、周期短、信噪比高、重復性好、細胞用量少，為極低起始細胞量和單細胞測序研究提供了可能性。自 2019 年 CUT&Tag 技術誕生以來，經過大量的實踐，發現該技術在組蛋白修飾及部分轉錄因子與 DNA 互作研究中可以獲得高信噪比的結果，但是針對一些與 DNA 結合力較弱或者結合具有高度動態性的 DNA 結合蛋白，較難得到理想的實驗結果。針對此問題，近岸蛋白開發了可對細胞進行高濃度甲醛交聯的CUT&Tag實驗流程。為轉錄因子 -DNA 互作研究提供一種新的思路，這種需要甲醛交聯的CUT&Tag實驗被稱為fcCUT&Tag（formaldehyde crosslinking CUT&Tag）。NovoNGS? CUT&Tag? 4.0 High-Sensitivity Kit (for Illumina）試劑盒包含常規非交聯細胞CUT&Tag及甲醛交聯細胞fcCUT&Tag實驗中所需的各種緩沖液、ConA磁珠、DNA純化磁珠、引物等全套建庫試劑，可以用于組蛋白修飾和轉錄因子的染色質結合狀態研究。與傳統的CUT&Tag試劑盒相比，該試劑盒既可以對自然狀態的細胞進行蛋白質-DNA互作研究，也可以對高濃度甲醛交聯的細胞進行蛋白質-DNA互作研究，一盒兩用。試劑盒包含CUT&Tag? 實驗中孵育抗體、轉座體、構建文庫的相關試劑，可以用于組蛋白修飾和轉錄因子的染色質結合狀態研究。本試劑盒包含高活性的ChiTag? pAG-Tn5, 相較于最初版本的pA-Tn5 轉座酶，pAG結構域對兔、鼠等多種來源抗體具有廣譜的強親和性，在實驗抗體的選擇上具有更大的空間。針對CUT&Tag?打斷產物DNA 提取過程中小片段丟失的問題，本試劑盒提供優化的Tagment DNA Extract Beads, 相較于常規的片段分選磁珠可以完整的回收CUT&Tag? 產物中的小片段DNA, 大大簡化了酚氯仿-乙醇沉淀法的繁瑣操作。此外，本試劑盒還提供了陽性抗體H3K4me3（兔抗）以及相應的二抗（羊抗兔IgG) ，用于陽性對照實驗（僅B 包裝提供陽性抗體）。圖1： DNA-Target Protein-Antibody-ChiTag?結合示意圖圖2： CUT&Tag 實驗流程簡圖產品用途DNA－蛋白質互作研究。在常見的哺乳動物細胞上用于替代傳統的ChIP-Seq 技術，特別適用于細胞數量較少的樣本，也可應用于經過處理的植物和酵母樣本上。保存條件詳見說明書僅供科研或生產使用，不可直接應用于人體。Novoprotein是蘇州近岸蛋白質科技股份有限公司的注冊商標。請訪問我們的注冊商標頁面。其他商標是其他所有者的財產。
Recombinant Human SHH 細胞因子
基本售價：840.00元
Recombinant Human SHH 細胞因子 產品描述Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197 is expressed.蛋白編號Q15465 產品別稱Sonic Hedgehog Protein; SHH; HHG-1分子量19.69 KDa表觀分子量19 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5.內毒素Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. (QC verified) 復溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產品背景Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.僅供科研或生產使用，不可直接應用于人體。Novoprotein是蘇州近岸蛋白質科技股份有限公司的注冊商標。請訪問我們的注冊商標頁面。其他商標是其他所有者的財產。
Recombinant Human FGF-10(Cat. No.:CR11)
基本售價：14400.00元
Recombinant Human FGF-10(Cat. No.:CR11) 該產品共有2篇文獻引用 產品描述Recombinant Human Fibroblast Growth Factor 10 is produced by our E.coli expression system and the target gene encoding Gln38-Ser208 is expressed.蛋白編號O15520 產品別稱Fibroblast growth factor 10;FGF-10;Keratinocyte growth factor 2;FGF10;KGF-2;KGF2分子量19.5 KDa表觀分子量19-22 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 8.0.內毒素Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. 純度-SEC-HPLCGreater than 95% as determined by SEC-HPLC. (Regularly tested) 生物活性-Cell Based AssayMouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#C029) , R-spondin 1 (Cat#CX83) ,FGF-10 (Cat#CR11) and HGF (Cat#CJ72). The organoids showed good morphology. 復溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產品背景Fibroblast growth factor 10 (FGF-10, KGF-2), is a member of the fibroblast growth factor (FGF) family that includes FGF-3, -7, and -22. KGF-2 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGFR2 and interacts more weakly with FGFR1. It plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. It exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 is required for normal branching morphogenesis. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.僅供科研或生產使用，不可直接應用于人體。
Recombinant Human RSPO1 (C-6His)(Cat. No.:CX83)
基本售價：17600.00元
Recombinant Human RSPO1 (C-6His)(Cat. No.:CX83) 該產品共有5篇文獻引用 產品描述Recombinant Human R-spondin-1 is produced by our Mammalian expression system and the target gene encoding Ser21-Ala263 is expressed with a 6His tag at the C-terminus.蛋白編號Q2MKA7 產品別稱RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof plate-specific spondin-1分子量27.8 KDa表觀分子量40 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.內毒素Less than 0.01 EU/μg as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. 生物活性-Cell Based AssayMeasured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 4.06 ng/ml. Mouse intestinal organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) and R-spondin 1 (Cat#CX83). After five days of culture, the organoids showed good morphology and germination rate. Mouse Colon organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , and R-spondin 1 (Cat#CX83). The organoids showed good morphology. Mouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#C029) , R-spondin 1 (Cat#CX83) ,FGF-10 (Cat#CR11) and HGF (Cat#CJ72). The organoids showed good morphology. Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#C687) , BMP-4(sample) , EGF (Cat#C029) , Wnt3a (Cat#C22R), Noggin (Cat#C028), R-spondin 1 (Cat#CX83) and FGF-4 (Cat#CR08) . The organoids showed good morphology. Human endometrial epithelial organoids were cultured with EGF (Cat#CH28) and R-spondin 1 (Cat#CX83). The organoids showed good morphology. Data were kindly provided by Novoprotein's customer. Thanks for her contribution. 復溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產品背景RSPO1 is a secreted protein,containing 2 FU(furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 is required for the early development of gonads, regardless of sex. It has been found in mice only eleven days after fertilization. To induce cell proliferation, it acts synergistically with WNT4. They help stabilize β catenin, which activates downstream targets. RSPO1 is necessary in female sex development. It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks after fertilization, the ovaries upregulate it while the testes downregulate it. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. This unfortunate condition often accompanies chemotherapy and radiation in cancer patients with head and neck tumors.僅供科研或生產使用，不可直接應用于人體。
Recombinant Human KGF(Cat. No.:CH73)
基本售價：19200.00元
Recombinant Human KGF(Cat. No.:CH73) 該產品共有1篇文獻引用 產品描述Recombinant Human Fibroblast Growth Factor 7/Keratinocyte Growth Factor is produced by our E.coli expression system and the target gene encoding Cys32-Thr194 is expressed.蛋白編號P21781 產品別稱Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF分子量18.9 KDa表觀分子量17 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM Tris，1mM EDTA，5% Trehalose, 0.02% Tween 80, pH 8.0.內毒素Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. (QC verified) 純度-SEC-HPLCGreater than 95% as determined by SEC-HPLC. (Regularly tested) 復溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產品背景Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.僅供科研或生產使用，不可直接應用于人體。
Recombinant Human PECAM-1 (C-6His)(Cat. No.:CS74)
基本售價：400.00元
Recombinant Human PECAM-1 (C-6His)(Cat. No.:CS74) 產品描述Recombinant Human Platelet Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln28-Lys601 is expressed with a 6His tag at the C-terminus.蛋白編號AAH51822.1 產品別稱Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA; PECA1; CD31; PECAM1分子量65.3 KDa表觀分子量75-110 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.內毒素 Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.復溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產品背景Platelet Endothelial Cell Adhesion Molecule (PECAM1, CD31), is a heavily glycosylated transmembrane protein belonging to the immunoglobulin (Ig) superfamily of cell adhesion molecules. CD31 is composed of an extracellular domain (ECD) of 574 amino acids (aa) containing six Ig-like domains, a transmembrane domain, and a 118 aa cytoplasmic domain. CD31 is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and natural killer (NK) cells. It is involved in cell adhesion and is required for transepithelial migration of leukocytes (TEM). CD31 acts as a homophilic receptor through its extracellular domain and is involved in downstream signaling via its cytoplasmic domain. This domain contains highly conserved ITIM motifs which, once tyrosine phosphorylated, recruit and activate the signaling molecules Src and SHP2. The resulting inhibition of TCR signaling increases the activation threshold of T cells, thus reinforcing peripheral tolerance and preventing development of autoimmunity. CD31 additionally regulates immune responses by acting as a key inhibitory receptor in dendritic cell development. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells.僅供科研或生產使用，不可直接應用于人體。
Recombinant Mouse IL-4 (C-6His)(Cat. No.:CK74)
基本售價：17600.00元
Recombinant Mouse IL-4 (C-6His)(Cat. No.:CK74) 該產品共有24篇文獻引用 產品描述Recombinant Mouse Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His21-Ser140 is expressed with a 6His tag at the C-terminus.蛋白編號P07750 產品別稱Interleukin-4; IL-4; IL4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; Lymphocyte stimulatory factor 1分子量14.6 KDa表觀分子量15-19 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.內毒素 Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.復溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產品背景Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.僅供科研或生產使用，不可直接應用于人體。
NovoStart SYBR qPCR SuperMix Plus試劑
基本售價：1280.00元
NovoStart SYBR qPCR SuperMix Plus試劑 該產品共有552篇文獻引用 產品描述本制品是采用SYBR Green I嵌合熒光法進行Real Time PCR的專用試劑。預混液中已經將DNA聚合酶、精心優化的反應Buffer、dNTPs、SYBR Green I等試劑預混在一起，是一種2×濃度的預混型試劑，進行實驗時，PCR反應液的配制十分簡單方便。本制品使用新型抗體修飾的HotStart Taq DNA聚合酶，并結合精心優化的反應Buffer，從而有效抑制低溫下的非特異性擴增，提高反應特異性和擴增效率，能夠在更寬廣的范圍內進行準確定量。產品特點高特異性：采用新型工藝制備抗體修飾的HotStart Taq DNA聚合酶，無需熱啟動即可進行PCR反應，大大提高PCR擴增的特異性。高效：Novoprotein精心配制的RealTime PCR專用2×SuperMix，具有更高的擴增效率和擴增靈敏度。快捷：PCR反應所必需試劑集于一管之中，數分鐘即可完成反應體系配制。保存條件-20℃避光儲存。如需在一段時間內經常取用，可在2~8℃條件下儲存3個月。避免反復多次凍融。質量控制純度檢測：所有組分經檢測均無核酸內切酶殘留、核酸外切酶殘留、核酸殘留。使用建議使用：請上下顛倒輕輕混合均勻，避免起泡，并經輕微離心后使用。反應液的配制、分裝請使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應體積為20～50μl。引物設計：一般為了增加靈敏度及擴增效率且抑制非特異性反應，擴增目標序列越短越好。建議設計成200bp以下。僅供科研或生產使用，不可直接應用于人體。
Recombinant Mouse TNF alpha
基本售價：2520.00元
Recombinant Mouse TNF alpha 產品描述Recombinant Mouse Tumor Necrosis Factor Alpha is produced by our E.coli expression system and the target gene encoding Asp89-Leu235 is expressed.蛋白編號P06804 產品別稱Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2分子量16.4 KDa表觀分子量14 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.內毒素Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.純度-SDS-PAGE
RNase R
基本售價：2000.00元
RNase R 產品描述核糖核酸酶R (Ribonuclease R，RNase R) 來源于大腸桿菌RNR超家族，是一種鎂離子依賴性的3'—5'核糖核酸外切酶，可從3'—5'方向將RNA逐步切割成二核苷酸和三核苷酸。RNase R幾乎能夠消化所有線性的RNA，但不易消化環狀RNA、套索結構RNA和3'端突出少于7個核苷酸的短雙鏈RNA分子。 RNase R常用于基因表達和可變剪切研究，可消化線性RNA以富集環狀RNA或套索結構RNA。保存條件-20±5℃僅供科研或生產使用，不可直接應用于人體。
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