溶葡萄球菌酶 (≥90%, ≥3000 unit/mg)  Lysostaphin from Staphylococcus simulans

別名：

CAS號：9011-93-2
分子量：27kDa
MDL：MFCD00131556
級別：BR
活性：≥3000 unit/mg solid
純度：≥90% (HPLC)
外觀/性狀：lyophilized powder
酶活性定義：One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.
等電點：10.5-11.0。
酶活性最適pH范圍：10～11。
影響因素：羥汞苯甲酸（PCMB）、 苯甲磺酰氯（PMSF）、二硫蘇糖醇（DTT） 等在1mmol/L的條件下，對酶活力無明顯影響，Zn離子表現(xiàn)出某種程度的活化效應；Ba離子、Mn離子、Ca離子、Mg離子和EDTA在1mmol/L的條件下，對溶葡球菌酶表現(xiàn)出一定程度的抑制作用
溶解性：可溶于50mmol/L Tris 緩沖液
來源：Recombinant, Expressed in E. coli.
用途：生化研究。專一降解金黃色葡萄球菌等細胞壁具有甘氨酸肽鍵結(jié)構(gòu)的細菌，通過裂解其細胞壁達到殺菌的目的。在pH值為4和5℃時高度穩(wěn)定。
產(chǎn)品描述：Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity.
A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus.
Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening. The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus.