一種重要的營養(yǎng)素和氧化還原劑，在骨鹽代謝及骨形成中具有重要作用。在體外，它能增加鈣鹽的沉積，促進(jìn)礦化結(jié)節(jié)的形成，并通過間充質(zhì)細(xì)胞間接調(diào)節(jié)破骨細(xì)胞的分化。維生素C能直接抑制核因子κB受體活化子配體（RANKL）誘導(dǎo)的破骨細(xì)胞形成 。    

使用本產(chǎn)品的應(yīng)用案例（僅供參考）

In Vitro

Cell（Osteogenic/成骨誘導(dǎo)；50 mg/l ascorbic acid）

The osteogenic medium was the complete culture medium supplemented with 10 nM dexamethasone(Solarbio), 10 mM β-glycerophosphate (Solarbio), and 50 mg/l ascorbic acid (Solarbio). 

數(shù)據(jù)來源文獻(xiàn)：Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821.

Cell（Osteogenic/成骨誘導(dǎo)；50 mg/l ascorbic acid）

Osteogenic induced medium contained complete culture medium supplemented with 100 nM dexamethasone (Solarbio, Beijing, China), 10 mM β-glycerophosphate (Solarbio) and 50 mg/l ascorbic acid (Solarbio).  The medium was replaced every other day.  Alizarin Red–positive mineralized matrix was used to evaluate osteogenic capacity by Alizarin red (AR) staining.

數(shù)據(jù)來源文獻(xiàn)：Zhao Y, Liu H, Xi X, Chen S, Liu D. TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. Exp Cell Res. 2020 Dec 1;397(1):112336. doi: 10.1016/j.yexcr.2020.112336. Epub 2020 Oct 19. PMID: 33091421.

In Vivo

Rat（雄性新生SD Rat；100 mg vitamin C/kg bw/d；灌胃；30d）

Forty-five male neonatal SD rats were randomly divided into five groups (n=9 each): corn oil control group, 500 mg DEHP/kg of body weight per day (bw/d) group, 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d group, 500 mg DEHP/kg bw/d+100 mg vitamin C/kg bw/d group, and 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d+100 mg vitamin C/kg bw/d group. DEHP and vitamin E (Solarbio, China) were dissolved in corn oil, and vitamin C (Solarbio,China) was dissolved in distilled water. From postnatal day (PND) 1 to 30, the male rats were administered corn oil, DEHP, vitamin C, and/or vitamin E by gavage each day according to their group. On PND 36, six rats of each groups were sacrificed and their testes were immediately isolated. The rest of rats were fed with routine feed until PND 90.

來源文獻(xiàn)：Tang X, Wu S, Shen L, Wei Y, Cao X, Wang Y, Long C, Zhou Y, Li D, Huang F, Liu B, Wei G. Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats. Environ Toxicol. 2018 Jul;33(7):720-728. doi: 10.1002/tox.22559. Epub 2018 Apr 16. PMID: 29663635.