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產品細節圖:
| 產品編號 | bs-4593R |
| 英文名稱 | MMP9 |
| 中文名稱 | 基質金屬蛋白酶9抗體 |
| 別 名 | Matrix metalloproteinase-9 precursor; MMP-9; MMP9; MMP 9; 92 kDa type IV; Collagenase; 92 kDa gelatinase; Gelatinase B; GELB; MMP9_HUMAN; 82 kDa matrix metalloproteinase-9; 92 kDa type IV collagenase; CLG 4B; CLG-4B; CLG4B; Collagenase Type 4 beta; Collagenase Type-4 beta; Collagenase type IV 92 KD; Collagenase type IV 92 KD; EC 3.4.24.35; Gelatinase 92 KD; Gelatinase 92 KD; Gelatinase beta; Gelatinase-beta; GelatinaseB; GELB; Macrophage gelatinase; MANDP2; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix Metalloproteinase 9; Type V collagenase. |
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Specific References (24) | bs-4593R has been referenced in 24 publications.
[IF=5.1] Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705. WB, IHC-P ; Dog.
[IF=4.776] Ma F et al. In situ fabrication of a composite hydrogel with tunable mechanical properties for cartilage tissue engineering. J. Mater. Chem. B, 2019. WB ; Human.
[IF=4.26] Parveen et al. Unconventional MAPK-GSK-3β Pathway Behind Atypical Epithelial-Mesenchymal Transition In Hepatocellular Carcinoma. (2017) Sci.Re. 7:8842 WB ; Human.
[IF=3.775] Chen Z et al. Final-2 targeted glycolysis mediated apoptosis and autophagy in human lung adenocarcinoma cells but failed to inhibit xenograft in nude mice. Food Chem Toxicol. 2019 Aug;130:1-11. WB ; Human.
[IF=3.457] Chai FN et al. Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122.Biomed Pharmacother. 2018 Jul;103:1002-1011. WB ; Mouse.
[IF=3.457] Li W et al. Upregulation of MMP-9 and CaMKII prompts cardiac electrophysiological changes that predispose denervated transplanted hearts to arrhythmogenesis after prolonged cold ischemic storage. Biomed Pharmacother. 2019 Feb 20;112:108641. WB ; Rat.
[IF=3.361] Varghese S et al. Immunostimulatory plant polysaccharides impede cancer progression and metastasis by avoiding off-target effects. Int Immunopharmacol. 2019 May 21;73:280-292. WB ; Human.
[IF=3.14] Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017). WB ; Human.
[IF=2.976] Chen X et al. Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells.(2019)Oncol Rep. Feb;41(2):765-778. WB ;
[IF=2.94] Deng, XiaHeng, et al. "MiR-21 involve in ERK-mediated upregulation of MMP9 in the rat hippocampus following cerebral ischemia." Brain Research Bulletin (2013). WB ; Rat.
[IF=2.54] Wang, Li, et al. "Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model." Peptides (2017). IHC-P ; Rabbit.
[IF=2.52] Liu, Tianbo, et al. "Correlation of TNFAIP8 overexpression with the proliferation, metastasis, and disease-free survival in endometrial cancer." Tumor Biology (2014): 1-10. IHC-P ; Human.
[IF=2.25] Yang X et al.Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cells. (2018)Biochem. Cell Biol. WB ; Human.
[IF=2.25] Shi S et al. MicroRNA-34a attenuates VEGF-mediated retinal angiogenesis via targeting the Notch1.(2018) Biochemistry and Cell Biology WB ; Rat &Human.
[IF=2.25] Fan S et al.Carboxypeptidase E-ΔN promotes migration, invasion and epithelial-mesenchymal transition of human osteosarcoma cell lines through the Wnt/β-catenin pathway.(2018) Biochem Cell Biol. WB ; Human.
[IF=2.11] Lee et al. Chondrocyte-derived extracellular matrix suppresses pathogenesis of human pterygium epithelial cells by blocking the NF-κB signaling pathways. (2017) Mol.Vi. 22:1490-1502 WB ; Human.
[IF=1.93] Lee, Hye Sook, et al. "Anti-neovascular effect of chondrocyte-derived extracellular matrix on corneal alkaline burns in rabbits." Graefes Archive for Clinical and Experimental Ophthalmology (2014): 1-11. IHC-P ; Rabbit.
[IF=1.68] Wang et al. The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment. (2017) J.Ophthalmol. 2017:6067891 IHC ; Rabbit.
[IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat.
[IF=1.466] Yan B et al. Suppression of puerarin on polymethylmethacrylate-induced lesion of peri-implant by inhibiting NF-κB activation in vitro and in vivo.Pathol Res Pract. 2019 Mar 1:152372. WB ; Mouse.
[IF=1.43] Wang, Xiao-yan, et al. "AMD3100 attenuates MMP-3 and MMP-9 expressions and prevents cartilage degradation in a monosodium iodoacetate-induced rat model of temporomandibular osteoarthritis." Journal of Oral and Maxillofacial Surgery (2016). IHC-P ; Rat.
[IF=1.39] He et al. Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2. (2018) Oncol.Lett. 15:3977-3984 WB ; Human.
[IF=0] Wang, Menglei, et al. "The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment." Journal of Ophthalmology 2017 (2017). IHC-P ; Rabbit.
[IF=0] Ye T et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019 Feb 6;38(1):55. FCM&IHC ; Mouse&Human.
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| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 信號轉導 轉錄調節因子 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Rabbit, Sheep, |
| 產品應用 |
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 78kDa |
| 細胞定位 | 細胞外基質 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP9:151-250/707 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 |
All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases). TIMPs have been shown to block tumor cell invasion suggesting that they act as metastasis suppressor genes. Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Subunit: Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Subcellular Location: Secreted, extracellular space, extracellular Tissue Specificity: Produced by normal alveolar macrophages and granulocytes. Post-translational modifications: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated. DISEASE: Defects in MMP9 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. Defects in MMP9 are the cause of metaphyseal anadysplasia type 2 (MANDP2) [MIM:613073]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Similarity: Belongs to the peptidase M10A family. SWISS: P14780 Gene ID: 4318 Database links: Entrez Gene: 4318 Human Entrez Gene: 17395 Mouse Omim: 120361 Human SwissProt: P14780 Human SwissProt: P41245 Mouse Unigene: 297413 Human Unigene: 4406 Mouse Unigene: 10209 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. MMP9亦稱IV型膠原酶或明膠酶B，其主要功能為降解IV型膠原。因而它在腫瘤細胞突破基底膜屏障和浸潤轉移中起重要作用。目前主要用于各種惡性腫瘤(如乳腺癌、胃腸道癌、卵巢癌、膀胱癌等)中的基底膜檢測與轉移浸潤的研究。細胞外基質在維持正常組織結構與功能以及細胞生長和分化過程中起重要作用。細胞外基質動態平衡的失調與腫瘤細胞侵襲、轉移和復發密切相關，基質金屬蛋白酶(MMP9)是細胞外基質的降解酶，可降解Ⅳ、Ⅴ、Ⅸ、Ⅺ型膠原，在腫瘤的浸潤、轉移過程中起重要作用，近年為腫瘤研究的熱點。 |
| 產品圖片 |
Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti-MMP9 (bs-4593R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 78 kD Observed band size: 70 kD |
暫時沒有說明書
1.《Multifunction Sr, Co and F co-doped microporous coating on titanium of antibacterial, angiogenic and osteogenic activities》
作者：Jianhong Zhou ,Lingzhou Zhao 影響因子：4.259
期刊：《Scientific Reports 6, Article number: 29069 (2016)》 PMID：27353337
作者：Jianhong Zhou ,Lingzhou Zhao 影響因子：4.259
期刊：《Scientific Reports 6, Article number: 29069 (2016)》 PMID：27353337
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